Precision assembly of nanoparticles, top-down meets bottom-up Thursday, 10 November 2016 EPFL researchers have developed a method to place and position hundreds of thousands of nanoparticles very precisely on a one centimeter square surface. This will open new doors in nanotechnologies. Whether it has to do with making pens or building space shuttles, the manufacturing process consists of creating components and then carefully assembling them. But when it comes to infinitely small structures, manipulating and assembling high-performance nanoparticles on a substrate is no mean feat. Researchers in EPFL’s Laboratory of... Read More...
Thousand of nanoantennas to enlighten living cells Friday, 22 May 2015 Large-scale arrays of photonic antennas for nanoscales dynamics in living cell membranes. A recent study carried out in collaboration with researchers of the EPFL and Thomas van Zanten, Mathieu Mivelle, and Carlo Manzo in the Single Molecule Biophotonics group led by ICREA Professor at ICFO Maria Garcia-Parajo, has succeeded in fabricating hundreds of thousands of photonic antennas to measure for the first time the nanoscale dynamics of individual molecules in living cells. This work constitutes a major breakthrough in our ability to... Read More...
Tracing the near field line Monday, 26 January 2015 Single Molecule Dipole Maps the Local Vector Field of an Optical Nano-antenna In a recent study, Anshuman Singh and Gaëtan Calbris of the Molecular NanoPhotonics group led by ICREA Professor at ICFO Niek van Hulst have used single molecule “point” dipoles and a scanning optical antenna to map the 3D vectorial antenna field with 1 nm resolution and to achieve enhanced fluorescence imaging with 40 nm FWHM response. The work, supported by EU project NanoVista, was published in NanoLetters. Read More...
Antenna-in-box platform to enhance single molecule detection Monday, 26 January 2015 Researchers from ICFO and CNRS publish results in Nature Nanotechnology. One of the ultimate goals of molecular biology is to watch how single molecules work at physiological conditions. This involves high local concentrations in the micromolar range, and calls for more than three orders of magnitude shrinking of the detection volume as compared to conventional optical microscopes. Hence, new nanotechnology tools need to be introduced in order to reach ultra-small detection volumes and turn single molecules into bright light sources. Read More...
The balance between adhesion and migration Monday, 26 January 2015 Re-activating adhesion on cells of the immune system in Plos One A recent single molecule study carried out by Kyra Borgman, Thomas van Zanten and Carlo Manzo in the Single Molecule Biophotonics group led by ICREA Professor at ICFO Maria Garcia-Parajo sheds new light on the tight balance between cell adhesion and migration in the immune system. The study reveals that integrin receptors (involved in controlling cell adhesion and migration) can be re-activated in mature dendritic cells and that their lateral mobility on the plasma... Read More...
Advances in the fields of molecular and cell biology are strongly coupled to the implementation of photonic tools that allow highly-sensitive measurements in living cells at high molecular concentrations and at the nanometer scale. The goal of NANO-VISTA is to exploit novel concepts of photonic antennas to develop a new generation of bionanophotonic tools for ultrasensitive detection, nanoimaging and nanospectroscopy of biomolecules, both in-vitro and in living cells. By taking advantage of the extraordinary field enhancement, directionality and nanofocusing of photonic antennas, our approach will allow single biomolecule detection in ultra-reduced detection volumes, including living cells.
The project focuses on three main objectives:
a) to pioneer the development of novel photonic antennas for ultrasensitive detection in fluids and simultaneous spatio-temporal superresolution in living cells;
b) to develop high-throughput large-scale nanofabrication of photonic antennas fully compatible with life science applications;
c) to demonstrate the functionality of the technology for biosensing and transferability into potential market products, and for nanoimaging and nanospectroscopy on living cells.
Thus, NANO-VISTA is fully targeted to the development of disruptive photonic technologies fundamental in strategic applications such as medicine and biology. To maximise the chances of success we have chosen for an interdisciplinary, trans-national and multi-institutional partnership (including a SME and a Medical Centre).
True European specialists, with long standing expertise in the fields of nanophotonics, photonic antennas, large-scale nanofabrication approaches and nanoimmunologists are concentrated in this proposal strengthening European research cohesion. In the mid-long term we expect that both, cell biologists as well as industrial sectors (biophotonic, microscopy and biotechnology enterprises) will benefit from this new technology.
The main concept of NANO_VISTA is summarised in the Figure below. Cutting edge fundamental research spanning the fields of nanophotonics, plasmonics and nanoimmunology are combined with novel routes of large scale nanofabrication technology to provide as end result a new photonic technology that will benefit both fundamental researchers in the Life Science field in general, as well as industrial sectors (biophotonics and nanotechnology enterprises to biosensing companies).
(Six) workpackages dedicated to RTD activities
WP1: Development of Photonic antenna probes for nanoimaging and nanospectroscopy on living cells
Coordinated byICFO (MNP group)
WP2: Development of novel2D photonic antenna arrays for biophotonic applications and biosensing
WP3: Large scale, high throughput nanofabrication of antenna arrays for life science applications
WP4: 2D antenna geometries for biosensing using controlled photon-electron generation in CMOS junctions
Coordinated by PixInBio (End M18)
WP5: Implementation of antenna geometries for nanoimaging & spectroscopy in live cells research
Coordinated byICFO (SMB group)
WP6: Generation of Biomolecular tool box and funtional consequences in cell response: health vs disease
WP9: Proto-type instrument exploiting 2D antennas
Coordinated byCOSINGO (START: M33)
WP 7: Dissemination and exploitation
WP 8: Management
Coordinated by ICFO
Each WP is headed by one member of the consortium. Block arrows denote intermediate deliverables of the project as well as input for other partner. WP1 & WP2 will explore & develop concepts for photonic antenna generation and are at the core of the technology. This will be further developed and implemented by WP3 and WP5. WP4, WP6 and WP9 are mostly dedicated to demonstration of improved functionality of the bionanophotonic technology. As such, the project crosses through fundamental physics, technology development and focussed targeted demonstration and impact. WP7 will be devoted to dissemination and exploitation activities while WP8 will ensure the adequate coordination between the WPs.